CBEMS 224 Molecular and Cellular Biophotonics (2014-2015)

CBEMS 224 Molecular and Cellular Biophotonics

(Not required for any major.)
Catalog Data:

CBEMS 224 Molecular and Cellular Biophotonics (Credit Units: 4) Principles underlying and application of photonics technologies to biomolecular and cellular systems. Sample technologies Optical Tweezers, Linear and Nonlinear Optical Microscopy and Fluorescence Lifetime and Correlation Methods and their use to investigate emergent problems in Molecular, Cellular, and Developmental Biology. Graduate students only. Same as CHEM 224. (Design units: 0)

Required Textbook:
Recommended Textbook:


Vasan Venugopalan
Relationship to Student Outcomes
No student outcomes specified.
Course Learning Outcomes. Students will:
Prerequisites by Topic

Requires graduate standing and permission of instructor.

Lecture Topics:
  • Lecture 1-1: Intro to Biological Microscopy -- Photophysical Fundamentals
  • Lecture 1-2: Biophotonic methods in Immunology
  • Lecture 2-1: Motor Proteins and Intercellular Transport
  • Lecture 2-2: Principles of Optical Tweezers
  • Lecture 3-1: Optical Tweezers Technology Fundamentals
  • Lecture 3-2: Student Reports/Presentations
  • Lecture 4-1: Fluorescence Microscopy for Study of Cellular Organization
  • Lecture 4-2: Principles of Fluorescence Microscopy
  • Lecture 5-1: Fluorescence Microscopy Technology Fundamentals
  • Lecture 5-2: Student Reports and Presentations
  • Lecture 6-1: Fluorescence Methods for Intercellular Dynamics
  • Lecture 6-2: Principles of Fluorescence Correlation and Lifetime Methods
  • Lecture 7-1: Technology Fundamentals for Advanced Fluorescence Methods
  • Lecture 7-2: Student Reports and Presentations
  • Lecture 8-1: Use of Biophotonic methods to study lipid metabolism
  • Lecture 8-2: Multi-photon, Second harmonic and Coherent Anti-Stokes Raman Microscopy
  • Lecture 9-1: Non-linear Microscopy Technology Fundamentals
  • Lecture 9-2: Student Reports and Presentations
  • Lecture 10-1: Application of Biophotonic Methods to Study of Cell Differentiation
  • Lecture 10-2: Course Wrap-Up
Class Schedule:

Meets for 3 hours of lecture each week for 10 weeks.

Computer Usage:

None beyond routine data analysis and image visualization/processing.

Laboratory Projects:


Professional Component


Design Content Description
Laboratory Portion:
Grading Criteria:

Problem Based Learning Projects: 35% Oral Presentations on Journal Papers: 35% Hands-on Practicum: 30%

Estimated ABET Category Content:

Mathematics and Basic Science: 0.0 credit units

Computing: 0.0 credit units

Engineering Topics: 0.0 credit units

Engineering Science: 0.0 credit units

Engineering Design: 0.0 credit units

April 23, 2014
Senate Approved:
March 25, 2014
Approved Effective:
2014 Fall Qtr